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Fig. 2. Two-hybrid interaction between XMog1 and Ran. The growth of yeast on plates with (His+) or without (His-) histidine is shown. Yeast were co-transformed with a plasmid encoding the activation domain of Gal4 fused to XMog1 (pAD-XMog1) and a second plasmid encoding the DNA-binding domain of GAL4 (pDB) fused to wild-type Ran, RanQ69L, RanT24N or p53 as a negative control. Co-transformation with Gal4AD/SV40 large T antigen and Gal4BD/p53 was used as a positive control. The diagram illustrates the position of each co-transformant on the plates.