Fig. 7. KNOLLE mRNA expression pattern in 35S::KN transgenic embryos and seedlings. In situ analysis using KN antisense probe. Embryos 8 µm, seedlings 17.5 µm sections. Unless stated otherwise, 35S::KN material is homozygous for the transgene. (A-H) embryos, (I-U) seedlings. (A) Wild-type, torpedo-stage embryo. (B) 35S::KN, torpedo-stage embryo. (C) Wild-type, bent-cotyledon stage embryo. (D) 35S::KN, bent-cotyledon stage embryo carrying only one copy of the transgene. Note slight KNOLLE mRNA overexpression in cotyledons. (E-H) 35S::KN, bent-cotyledon stage embryo. KNOLLE mRNA is overexpressed in cotyledons. (E) Overview; (F) higher magnification of area boxed in E — expression in pre-vasculature of cotyledon (arrowheads). (G) Overview; (H) higher magnification of area boxed in G — arrowheads indicate cells with stronger signals resembling the patchy mRNA pattern observed in wild type. (I) Wild-type, root with root hairs. (J) 35S::KN, root with root hair. KN mRNA is accumulated in central part of the root. (K) 35S::KN, lateral root primordium with strongly labelled cells (asterisks) and young root hair (arrowhead) show mRNA accumulation. cot, cotyledon. Scale bars: 50 µm.