Fig. 7. Inhibition of PYK2 autophosphorylation by FAK. (A) FAK N-terminal domain is required for inhibition of PYK2 autophosphorylation. (B) FAK tyrosine phosphorylation was not required for inhibition of PYK2 autophosphorylation. HEK 293 cells were transfected with constructs encoding Myc-tagged PYK2, PYK2 plus FAK or PYK2 plus FAK mutants as indicated. Cell lysates were immunoblotted with anti-Myc antibodies to detect the production of PYK2, FAK or FAK mutants and anti-phosphotyrosine 402 (PY402, Biosource). Solid arrows indicate PYK2 and open arrows indicate FAK or FAK mutants.