Fig. 2. Alterations of cell morphology and focal adhesions in Swiss 3T3 cells expressing PYK2. Swiss 3T3 fibroblasts were microinjected with plasmids encoding Myc-tagged PYK2 (A-C,G-I) or FAK (D-F,J-L). Cells were fixed for 2-4 hours after injection and immunostained with anti-PYK2 (polyclonal, against PYK2 amino acids 587-988) (A,G) and anti-vinculin (monoclonal) (B,E) or anti-FAK (monoclonal) (D,J) antibodies, or with phalloidin (H,K); C, F, I and L are overlay pictures. Bar, 50 µm.