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Fig. 9. CENP-E staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-E staining in B6 early prometaphase (A,B) and late prometaphase spermatocytes (C,D) (ß-tubulin staining in red, CENP-E staining in green). (A,C) Overlays of CENP-E and ß-tubulin staining; (B,D) CENP-E staining only. (E-G) CENP-E (green) staining in a Rb/+ MI spermatocyte, containing misaligned chromosomes (arrowheads). (E) CENP-E staining is in green and (F) DAPI staining (for DNA) is in blue; (G) overlay of E,F with MPM-2 staining in orange. Scale bars: 10 µm.