JCS -- Ochotorena et al. 114 (16): 2911 Figure IG4

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Fig. 4. The wat1 mutant shows reduced ${alpha}$ -tubulin levels. (A) Reduction of ${alpha}$ -tubulin levels but not other proteins. Protein extracts were prepared from exponentially growing wild-type or wat1-5235 strain, run on SDS-PAGE and immunoblotting was performed with various antibodies shown. (B) Decrease of ${alpha}$ -tubulin levels upon upwards temperature shift. Wild-type (lanes 1, 4 and 7), wat1-5235 (lanes 2, 5 and 8) or wat1-deleted cells (lanes 3, 6 and 9) were grown at 27°C (lanes 1 to 3) and shifted up to 36°C. After 2 (lanes 4 to 6) and 4 hours (lanes 7 to 9), protein extracts were prepared and immunoblotting was performed with anti- ${alpha}$ -tubulin (top) or anti-Cdc2 antibody (bottom).