Fig. 6. K1735 cell migration requires active Src. (A) M2, M2KD-Src, C23, and C23CA-Src cells were analyzed for autophosphorylation of Src by kinase assay (top-left). Immunoprecipitation followed by western blotting using anti-Src antibodies was done to detect changes in expression as a result of transduction with the mutant constructs (bottom-left). Western blotting of whole cell lysate was done to detect changes in Src expression as a result of transduction with the Src constructs (top-right). Western blotting of whole cell lysate with antibodies to paxillin was used as protein loading control (bottom-right). (B) Migration of the four cell lines was evaluated on VN. The results (means±s.e.m.) are representative of three independent experiments each performed in triplicate.