Fig. 11. flp1⁺ functions in the S. pombe morphology checkpoint. Cells of the indicated genotype were grown at 25°C in YE, then shifted for 5 hours to 36°C before fixation. (A,B) DAPI and Calcofluor staining of cells 5 hours after shift to 36°C. (C) The percentage of cells with one, two or more than two nuclei. Cells were scored as 2 or >2 nuclei, whether they were septated or not, as long as there was no sign of cleavage of the septum.