Fig. 8. The RS domain of CrkRS is required for its localisation to nuclear speckles. (A) Schematic diagram of the CrkRS deletion constructs (i-x). The YFP tag (green), kinase domain (red) proline-rich regions (blue), RS domain (grey) and the bipartite nuclear localisation sequences (stars) are shown. Constructs (i-x) were microinjected into HeLa cells and the subcellular localisation of each fusion protein assayed by confocal laser scanning microscopy. (B) Representative fluorescence images. Each image is representative of 60 microinjected cells 4-6 hours after microinjection. Constructs were assayed twice in two separate experiments.