Fig. 7. CrkRS is an MPM-2 antigen. (A) Immunoblot. Anti-CrkRS immunoprecipitates from interphase and mitotic HeLa cell fractions were incubated in buffer alone, with protein phosphatase, or with protein phosphatase plus phosphatase inhibitors, and immunoblotted with the MPM-2 mAb (top) or with anti-CrkRS antibodies (bottom). CrkRS was recognised by MPM-2 mAbs in a phosphorylation-dependent manner. (B) CrkRS and MPM-2 colocalise during interphase. HeLa cells were fixed and stained with MPM-2 mAb (left) and anti-CrkRS antibodies (middle). The patterns overlap in the nuclear speckles (Merge).