Fig. 5. CrkRS localises to SC35 nuclear speckles. (A) HeLa cells were fixed and immunostained with affinity-purified anti-CrkRS antibodies (i), precleared affinity-purified anti-CrkRS antibodies (ii) or control rabbit IgG (iii) and visualised by confocal laser scanning microscopy. (B) CrkRS mainly colocalises with SC35. To verify that CrkRS localises to nuclear speckles, cells were costained with anti-CrkRS antibodies and antibodies against SC35, a known nuclear speckle protein. The merged image shows the extent of overlap between CrkRS and SC35.