Fig. 9. The formation of spacious phagosomes is not blocked by overexpression of DN Rab7. Control cells and cells overexpressing DN Rab7 were fed a mixture of FITC-labeled bacteria and Crimson Red latex beads for 10 minutes, washed and then chased for 120 minutes. During the last 10 minutes of chase, cells were allowed to attach to coverslips and then imaged by LSCM, as described in Materials and Methods. The mean number of phagosomes labeled by both crimson red beads and FITC-bacteria per cell were plotted (n=3). Values are means ± s.e.m. P<0.05 for X4 versus LY294002. X4, control cells; 7TN, cells overexpressing DN Rab7; LY294002, cells incubated in the presence of 20 µM LY294002.