Fig. 9. Effect of a microtubule-destabilizing agent on the centromere clustering. Zygotes of the cdc25 mutant (BG992) were transferred into the rich medium and 2 hours later the incubation temperature was raised to 35°C (time 0). Cells were stained with DAPI (Chr), Cy3-labelled pRS140 (Cen) and anti-Sad1 antibody (Sad1). Merged images: white, DAPI; green, centromeres; red, Sad1. (A) 0 hours, (B-D) 3 hours after transfer. YE (B) or DMSO (C) was added as a control. (D) Cells were treated with 100 µg/ml of thiabendazole (TBZ). (E) Number of cells with clustered or scattered centromeres. Scattered centromeres are present in at least two sites. (F) In a separate experiment, TBZ was added 3 hours after the temperature shift and centromere clustering was observed 1 hour later. (E,F) Red, presumed SPB; green, centromeres. *This number includes two nuclei with clustered centromeres but detached from the SPB.