
Fig. 4. Association of the Sad1-bodies with cytoplasmic microtubules. Wild-type zygotes (BG991) were stained with DAPI (Chr), anti-Sad1 antibody (Sad1) and TAT1 (MT, microtubule). Merged: blue, DAPI; green, MT; and red, Sad1. (A,B) 3 hours, (C) 4 hours after the transfer. A spindle formed during RTG is shown in C. Bar, 2.5 µm.