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Fig. 10. Spindle dynamics and chromosome segregation in the first mitosis during RTG. Live observation of spindle elongation in RTG (B). (A) Spindles in ordinary mitoses of diploid cells were measured. (C,D) Typical images of early-to-late anaphase of the first mitosis. Chr, DAPI; cos1228, Cy3-labelled cos1228; MT, TAT1/Cy5; Cen, Cy5-labelled pRS140; Merged: white, DAPI; red, cos1228; green, TAT1 (C) and pRS140 (D).