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Fig. 5. SCP2/ SCP3 localization at diakinesis/metaphase I in spermatocytes and oocytes. (A,C) Chromosomes (blue) immunostained with CREST antiserum, which localizes to the centromeres (green). (B,D) Chromosomes (blue) and SCP3 localization (red). (A,B) Two spermatocytes at diakinesis/MI exhibiting SCP3 localization to all centromeres. (C,D) A comparably fixed oocyte showing CREST staining but no SCP3 localization. (Note: the localization pattern of SCP2 is identical.)