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Fig. 5. Lte1 undergoes cell-cycle-dependent phosphorylation that is maintained following nocodazole treatment. Strain SJY121 (LTE1-3HA) was synchronised with {alpha}-factor and released into fresh medium at 25°C in the absence (A) or presence (B) of nocodazole. Samples for protein extraction were taken at the indicated times after release. Immunoblots were analysed with anti-HA or anti-Clb2 as indicated. The lower panel shows budding curves for the two cultures.