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Fig. 4. Bfa1 undergoes cell-cycle-dependent phosphorylation that is enhanced following nocodazole treatment. Strain SLY105 (3HA-BFA1) was synchronised with {alpha}-factor and released into fresh medium in the absence (A) or presence (B) of nocodazole. Samples for protein extraction were taken at the indicated times after release. Extracts were immunoblotted and probed with anti-HA or anti-Clb2 as indicated. The right panel shows budding curves for the two cultures.