Fig. 5. Immunofluorescence micrographs of transfected corneal fibroblasts reacted with anti-myc antibody 9E10. Fibroblasts transfected with constructs HM and D13 showed predominantly cytoplasmic signals for myc-tagged ferritin, whereas cells transfected with construct D11 showed both nuclear and cytoplasmic signals (top row). When the NLS from the SV-40 large T antigen was attached to these constructs, yielding HM-NLS, D13-NLS and D11-NLS, their products underwent nuclear translocation (bottom row). Bar, 10 µm.