Fig. 3. Immunofluorescence micrographs of transfected day-14 corneal epithelial cells reacted with anti-myc antibody 9E10. The transfected constructs in the top row (HM, D11 and D13) showed predominantly nuclear immunoreactivity. The constructs in the middle row (D10, DL and DD) showed predominantly cytoplasmic immunoreactivity; however, these constructs were capable of undergoing nuclear translocation if the NLS of SV-40 large T antigen was added to them, yielding the bottom row (D10-NLS, DL-NLS, DD-NLS). Bar, 10 µm.