Fig. 6. Epidermal CeAJs are heterogeneous structures in wild-type embryos. (A) Schematic diagram of part of an embryo at the beginning of elongation indicating the positions of the projected transverse sections (blue and tan brackets) displayed in (B) and in Fig. 7C. (C,D) Z-axis rotations of transverse sections through the epidermis along the membrane of two connecting epidermal cells where the CeAJ is visible as a subapical belt, as illustrated in B. In B-D, asterisks denote epithelial nuclei on either side of the membrane in question. (C) dlg-1::gfp transgenic embryo after immunostaining with MH27 (anti-JAM-1), anti-GFP and anti-LIN-26 antibodies; (D) jam-1::gfp transgenic embryo after immunostaining with anti-HMP-1, anti-GFP and anti-LIN-26 antibodies. DLG-1 and JAM-1 appear to colocalise completely (C) while HMP-1 and JAM-1 appear to be mutually exclusive in their localisation (D; yellow regions may result from limited resolution). (E-G) Wild-type, dlg-1(RNAi) and let-413(RNAi) embryos, respectively, at 8-11 hours development after immunostaining with MH27. (H-J) Wild-type, dlg-1(RNAi) and let-413(RNAi) embryos, respectively, at 8-11 hours development after immunostaining with anti-HMP-1 antibodies. It can be seen that the JAM-1 belt around epidermal cells can be altered independently of the HMP-1 belt. Scale bars, 5 µm (C,D); 10 µm (E-J).