Fig. 1. dlg-1(RNAi) embryos show abnormal morphogenesis and epithelial defects. (A-D) Nomarski interference microscopy of wild-type and dlg-1(RNAi) embryos. (E-H) Confocal images showing immunostaining with the monoclonal antibody MH27, which recognises CeAJs in the epidermis, pharynx (arrows) and intestine (arrowheads). (C,E,G) External focal plane showing the epidermis. (A,B,D,F,H) Internal focal plane showing the pharynx and intestine. (A,E,F) 1.5-fold stage wild-type embryos (6-7 hours development). (B) Wild-type pretzel stage embryo (9-10 hours development). (C,G,H) dlg-1(RNAi) embryos at 6-7 hours development. (D) dlg-1(RNAi) embryos at 9-10 hours development. In dlg-1(RNAi) embryos, CeAJ staining is punctate and worsens with age; epidermal CeAJs are more abnormal than in the pharynx or intestine. In C and D, white arrows indicate epithelial vacuoles. Also in C a black arrowhead indicates abnormal bulges at the embryo surface and in D a white arrowhead indicates loose cells that have leaked out of the embryo. In this and all following figures unless otherwise stated, the embryos are orientated with the anterior to the left and dorsal to the top of the image. Scale bar,10 µm.