Fig. 7. The FYVE mutation R183A inhibits membrane targeting of Hrs. (A) BHK cells were transfected with myc-tagged Hrs^R183A. The cells were stained with anti-myc (left) and anti-EEA1 (middle). Yellow colour in the merged images (right) indicates colocalisation. Nuclei are stained with Toto-3 (blue). Bar, 5 µm. (B) BHK cells were transfected with myc-tagged wild-type Hrs or Hrs^R183A. Equivalent samples of the post-nuclear supernatant (PNS), cytosol (C) and membrane (M) fractions were analysed by SDS-PAGE and immunoblotting with anti-myc antibodies.