Fig. 3. The endosomal localisation of EEA1 but not Hrs is abolished upon expression of a dominant-negative Rab5 mutant. BHK cells were transfected with myc-Rab5^S34N and permeabilized with 0.05% saponin (Simonsen et al., 1998a) prior to fixation. They were then stained with anti-myc (A,C), anti-EEA1 (B) or anti-Hrs (D). Transfected cells are indicated by arrows. Bar, 5 µm.