Fig. 8 Strains CJY49-3-4 (wildtype), CJY62-10-3 gea1-4 and APY022 gea1-6 were grown at 24°C, then shifted to 37°C for 10 minutes. Cells were spun down, resuspended in a small volume of medium prewarmed to 37°C, and FM4-64 added at time 0. Cells were incubated at 37°C, and at the time points shown (minutes after addition of FM4-64), cells were removed and fixed with formaldehyde. Bar, 5 µm.