Fig. 6. Localization of Anp1p and Och1-HA in gea mutants. Strains CJY62-10-3 gea1-4/pOH (A) and APY022 gea1-6/pOH (B) were grown at 24°C, and either shifted to 37°C for 40 minutes, or left at 24°C. Cells were fixed and prepared for immunofluorescence analysis using rabbit affinity-purified anti-Anp1p antiserum and mouse monoclonal anti-HA antibodies. Arrows indicate ring-like structures. Bar, 5 µm.