Fig. 5. Localization of Och1-HA in wildtype and gea mutant strains. The wild-type strain CJY49-3-4 (A), strain CJY62-10-3 gea1-4 (B) and strain APY022 gea1-6 (C), each carrying plasmid pOH (Och1-HA), were grown at 24°C, and either shifted to 37°C for 40 minutes, or left at 24°C. Cells were fixed and prepared for immunofluorescence analysis using monoclonal anti-HA antibodies. Left panels, DAPI staining to visualize nuclei; right panels, anti-HA. Arrows indicate ring-like structures and arrowheads ER/nuclear envelope staining. Bar, 5 µm.