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Fig. 3. (A) Strains CJY49-2-3 and CJY054 were preincubated for 45 minutes at 38°C, labeled for 10 minutes and chased for 30 minutes, all at 38°C. Cells and medium were separated and HSP150 immunoprecipitated using polyclonal anti-HSP150 antiserum. (B) Strains CJY52-10-2/pCLJ90-GEA1 and CJY052-10-2/pNTS19-gea1-19 were preincubated for 30 minutes at 37°C, labeled for 10 minutes and chased for 10 minutes, all at 37°C. Cells were then converted to spheroplasts, and internal and external fractions immunoprecipitated with antibodies against invertase. (C,D) Strains CJY52-10-2/pCLJ90-GEA1 and CJY052-10-2/pNTS19-gea1-19 were preincubated for 30 minutes at 37°C, labeled for 10 minutes and chased for the amount of time indicated. Cell extracts were immunoprecipitated with anti-ALP1 monoclonal antibodies (C) or with anti-{alpha}-pheromone antiserum (D), and immunoprecipitates analyzed by SDS-PAGE.