Fig. 4. erg6 strain treated for 5 minutes with BFA. (a) A tubular Golgi network with small dilations at the intersections of polygonal meshes (G) is seen next to a curved sheet of unperforated ER (er). At the left, a tubular network (white arrow) is seen in profile and is continuous with a portion of subplasmalemmal ER at the top-left. A small vacuole-like structure (V) is located next to the nucleus (N). Magnification x39,700. (b) Several small vacuole-like structures of variable staining densities (V) are in close contact with the nuclear envelope. At the bottom-left, one of these structures is continuous with an ER sheet (ER). A large intensely stained vacuole (*) is seen proximal to the nucleus (N). Magnification x38,500. (c) Next to the nucleus (N) and connected to a subplasmalemmal ER sheet (ER), there is a large tubular network (arrow) forming an ovoid mass. Light and dense vacuoles are also labeled (V). Magnification x40,100. (d) A tubular Golgi network with small dilations at the intersections of narrow, irregular meshes (G) is continuous at one side with a wide-meshed tubular network (arrow) and is connected on the right with a parallel array of anastomosed tubules (white arrow). N, nucleus. Magnification x43,350. (e) A parallel array of saccules (white arrow) is seen in proximity to an ER sheet (ER). A large vacuole is labeled (V). Magnification x82,100.